Extracellular pH dynamics of retinal horizontal cells examined using electrochemical 1 and fluorometric methods 2 Jason Jacoby 1 , Matthew
نویسندگان
چکیده
Boriana K. Tchernookova, Ethan R. Naylor, 4 Robert Paul Malchow 5 6 1 Department of Biological Sciences, University of Illinois at Chicago, Chicago, IL, USA 7 2 Department of Biology, Indiana Wesleyan University, Marion, IN, USA 8 3 National Eye Institute, Bethesda, MD, USA 9 4 Department of Ophthalmology and Visual Sciences, University of Illinois at Chicago, 10 Chicago, IL, USA 11 12
منابع مشابه
Extracellular pH dynamics of retinal horizontal cells examined using electrochemical and fluorometric methods.
Extracellular H(+) has been hypothesized to mediate feedback inhibition from horizontal cells onto vertebrate photoreceptors. According to this hypothesis, depolarization of horizontal cells should induce extracellular acidification adjacent to the cell membrane. Experiments testing this hypothesis have produced conflicting results. Studies examining carp and goldfish horizontal cells loaded wi...
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35 36 37 Extracellular acidification induced by retinal horizontal cells has been 38 hypothesized to underlie lateral feedback inhibition onto vertebrate 39 photoreceptors. To test this hypothesis, the H-sensitive fluorophore HAF (540 hexadecanoylaminofluorescein) was used to measure changes in H from 41 horizontal cells isolated from the retina of the catfish. HAF staining conditions 42 were m...
متن کاملFluorescent imaging reports an extracellular alkalinization induced by glutamatergic activation of isolated retinal horizontal cells.
Extracellular acidification induced by retinal horizontal cells has been hypothesized to underlie lateral feedback inhibition onto vertebrate photoreceptors. To test this hypothesis, the H(+)-sensitive fluorophore 5-hexadecanoylaminofluorescein (HAF) was used to measure changes in H(+) from horizontal cells isolated from the retina of the catfish. HAF staining conditions were modified to minimi...
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Negative feedback from horizontal cells to cone photoreceptors is regarded as the critical pathway for the formation of the antagonistic surround of retinal neurons, yet the mechanism by which horizontal cells accomplish negative feedback has been difficult to determine. Recent evidence suggests that feedback uses a novel, non-GABAergic pathway that directly modulates the calcium current in con...
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It was demonstrated in our recent study that Zn(2+) had dual effects on Ca(2+)-permeable AMPA receptors on carp retinal horizontal cells. However, the possible mechanism(s) underlying the Zn(2+) effects is still unclear. In the present study, we examined the Zn(2+) effects in the presence of cyclothiazide (CTZ), an allosteric potentiator of AMPA receptors, which could attenuate the receptor des...
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